Asymptomatic Leishmania Infection in Human Population of Wenxian County,Gansu Province
- VernacularTitle:甘肃省文县流行区人群婴儿利什曼原虫无症状感染现状
- Author:
Junyun WANG
;
Yu FENG
;
Chunhua GAO
;
Changfa JIN
;
Shengbang CHEN
;
Chouji ZHANG
;
Jinping HE
;
Chenming YANG
;
Yuetao YANG
;
Yifang BAO
- Publication Type:Journal Article
- Keywords:
Leishmania infantum;
Asymptomatic infection;
PCR;
ELISA;
rK39-dipstick
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the status of Leishmania infantum asymptomatic infection in human population of a Kala-azar endemic area in Wenxian County,Gansu Province,and to evaluate the tests used.Methods Blood samples were tested by PCR using two pairs of primers,RV1-RV2 and K13A-K13B,for detecting Leishmania-specific DNA.ELISA and rK39-dipstick were used to detect Leishmania-specific antibodies.Results The positive rate of PCR,ELISA and rK39-dipstick was 30.9%(83/269),24.2%(65/269) and 0(0/269) respectively.Conclusion The prevalence of asymptomatic infection of L.infantum in humans is high in the area.PCR test based on RV1-RV2 and K13A-K13B primer pairs is a sensitive and specific method for detecting the asymptomatic infection.
