Protective effect of the new type of Tongxinluo on blood-brain barrier impairment after cerebral ischemic/reperfusion injury in rats
- VernacularTitle:新型通心络制剂对大鼠脑缺血再灌注后血-脑屏障损伤的保护作用
- Author:
Songdi WU
;
Qi WAN
;
Feng XIA
- Publication Type:Journal Article
- Keywords:
blood-brain barrier;
cerebral ischemia/reperfusion;
S100? protein;
Tongxinluo
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of the new type of Tongxinluo (TXL) on blood-brain barrier impairment after cerebral ischemic/reperfusion injury in rats.Methods 78 male Sprague-Dawley rats were randomly divided into sham-operated group (n=6), operated group (n=36) and TXL-treated group (n=36), the latter two groups were divided into 0.5 h, 2 h, 6 h, 12 h, 24 h and 48 h groups (n=6, each) according to the time after cerebral ischemic/reperfusion for 2 h. The rats were pretreated with normal saline or TXL for 7 d. The middle cerebral artery occlusion/reperfusion (MCAO/R) rat model was established by the Longa occlusion method;The serum S100? protein concentrations of different point time were determined by ELISA method and infarct volumes were determined by 2,3,5-triphenyltetrazolium Chloride (TTC) staining method. Results (1) The serum S100? protein level in TXL-treated groups were significantly decreased compared with operated groups at 0.5 h, 2 h, 6 h,12 h and 24 h after MCAO/R (P
