Effects of large dose Methylprednisolone on level of MCP-1 expression and macrophages infiltration in early spinal cord injured rats
- VernacularTitle:大剂量甲基泼尼松龙对大鼠脊髓损伤早期单核细胞趋化蛋白-1表达水平和巨噬细胞浸润的影响
- Author:
Shengzhong MA
;
Jiwen TANG
;
Xuesong ZHANG
- Publication Type:Journal Article
- Keywords:
spinal cord injury;
Methylprednisolone;
MCP-1;
macrophage
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Methylprednisolone (MP) on level of monocyte chemoattractant protein 1 (MCP-1) expression and macrophages infiltration in early spinal cord injured (SCI) rats.Methods Animal SCI model was established by modified Nystrm method. 72 rats were randomly divided into three groups: SCI group, normal saline control group and MP treatment group. Each group,divided into three subgroups, were sacrificed at 1 d, 3 d, 7 d postinjury. Spinal cord tissues were collected and sections were made to do following examinations: (1)morphological observation by hematoxylin and eosin stain;(2)the expression and distribution of local MCP-1 positive cells, and the infiltration of macrophages by immunohistochemical method. The cell population of different time points were counted and compared.Results MP reduced the proportion of both MCP-1 positive cells and marcrophages in local spinal cord, meanwhile MP elevated the mean density of Olsiwski corpuscle at all time points as compared with those in control group (all P
