Human lung carcinoma cell A549 express vascular endothelial grouth factor-C induced by hyaluronan
- VernacularTitle:透明质酸诱导人肺腺癌A549细胞表达血管内皮生长因子-C
- Author:
Wenlei ZHUO
;
Yan WANG
;
Zhengtang CHEN
;
Xujun AO
- Publication Type:Journal Article
- Keywords:
Hyaluronan;
Lung carcinoma;
Vascular endothelial growth factor-C;
A549 cell
- From:
Journal of Medical Postgraduates
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of VEGF-C in human lung carcinoma cell A549 induced by hyaluronan(HA) in vitro. Methods:A549 cells cultured in vitro were divided into three groups: control group(C): cultured with free serum medium(FSM);HA treatment group 1(HA1) and group 2(HA2): cultured with FSM containing different concentration of HA(HA1 group: 10?g/ml,HA2 group: 20?g/ml).After 48 hours,VEGF-C expression were examined by reverse transcription-polymerase chain reaction(RT-PCR)? Western blot and immunofluorescence staining. Results:Group C: both VEGF-C mRNA and protein expression were weak positive.HA1 and HA2 groups: both VEGF-C mRNA and protein expression were strong positive and increased significantly in a HA dose-depended way(P
