Effect of human umbilical cord blood adherent cells on the expansion of umbilical cord blood hematopoietic cells in vitro
- VernacularTitle:人脐血贴壁层细胞对脐血造血细胞体外扩增的作用
- Author:
Xiaohong JU
;
Zhenyi ZHAO
;
Fang FANG
;
Aixin MA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
The experiment was performed at the Institute of Treatment and Prevention of Tumor of Jilin Province and Department of Etiology, Jilin Medical College from October 2004 to June 2005. The normal umbilical cord blood (UCB) was provided by a healthy lying-in woman from Changchun Hospital with the permission of the pregnant woman. Full-term normal delivery and UCB of healthy pregnant women was collected by aseptic venepuncture after fetal disengagement, and with Natrium Citricum anticoagulation. After the UCB mononuclear cells were isolated by Ficoll-paque (relative volume mass: 1.077?0.22) density gradient centrifugation, long-term liquid culture system was used for UCB adherent cells, and co-cultured with UCB mononuclear cells. The adherent cells were observed and mononuclear cell cycles were tested with flow cytometer. It showed that adherent layer of UCB increased UCB mononuclear cells expansion as compared with that of non-adherent layer of UCB after co-culture for 7 days. The cell percentage in S+G2+M phase obviously increased, and there was significant difference [(42.7?1.1)%,(35.5?2.8)%,P
