Ooplasmic transfer, cloning and reverse cloning
- VernacularTitle:卵胞质移植、克隆与逆向克隆
- Author:
Xinxia QIU
;
Yinghua CHEN
;
Binglei ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: Inter-oocyte cytoplasmic transfer has been performed recently as a means to improve the outcome of assisted reproductive methods and becomes a hotspot. To overview the recent advances and problems in this area in this article, and put forth a new concept of reverse cloning technique based on ooplasmic transfer. DATA SOURCES: An online search of PUBMED was undertaken to identify correlative English articles published from January 1998 to December 2006 by using the keywords of "ooplasmic transfer, mitochondria heteroplasmy,animal cloning". Meanwhile, relevant Chinese articles published between January 1998 and December 2005 were searched in Wanfang database with the same keywords in Chinese. STUDY SELECTION:The articles were primarily screened, and the citations at the end of each reference were reviewed. Inclusion criteria: The articles related to the research of ooplasmic transfer, cloning or reverse cloning. Exclusion criteria: Repetitive studies or Meta analytical articles. DATA EXTRACTION:Totally 126 relevant articles were collected, and 31 of them were in accordance with the inclusion criteria, while 95 of them were excluded because of repetition or obsolescence. In 31 articles met the criteria, 17 studies were relevant to the ooplasmic transfer, 13 studies related to animal cloning and one study related to reverse cloning. DATA SYNTHESIS: Exciting advances have been achieved in human gamete biology, and ooplasmic transfer have been widely applied. Many studies indicated that mitochondria in oocytoplasm affected obviously the fertilization of the oocytes and early embryo development. Lots of experiments have been done on the interaction of cytoplasm and nucleus as well as the mechanism of genomic reprogramming to further the investigation. Meanwhile, the techniques are progressed. Cloning techniques is excellent in application prospect, while there are some problems at the same time. Therefore the concept of reverse cloning technique was proposed based on ooplasmic transfer. CONCLUSION: The mitochondria in oocytoplasm has remarkable effect on the fertilization of the oocytes and early embryonic development. However, ooplasm transfer can lead to mitochondria DNA heteroplasmy, and more work must be done on potential problems. The reverse cloning technique based on the ooplasmic transfer has a good prospect.
