Screening of EGFR-regulated Secreted Proteins in Human NPC Cell Line CNE2
- VernacularTitle:表皮生长因子受体调控的人鼻咽癌细胞系CNE2分泌蛋白质的筛选
- Author:
Ke LIANG
;
Zhuchu CHEN
;
Hong YI
;
Jianling LI
;
Pengfei ZHANG
;
Maoyu LI
;
Cui LI
;
Xueping FENG
;
Fang PENG
;
Zhiqiang XIAO
- Publication Type:Journal Article
- Keywords:
nasopharyngeal carcinoma (NPC), CNE2, epidermal growth factor receptor (EGFR), secretome, two-dimensional gel electrophoresis, mass spectrometry
- From:
Progress in Biochemistry and Biophysics
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to screen EGFR-regulated secreted proteins in human nasopharyngeal carcinoma(NPC), and to reveal the role and mechanism of epidermal growth factor receptor(EGFR) in the pathogenesis of NPC. NPC cell line CNE2 cells were cultured in serum-free medium and stimulated by transforming growth factor-? (TGF-?) for 24 h in experimental group. Control CNE2 cells were cultured at the same condition but without TGF-? stimulation. The culture medium of control and experimental cells was desalted and concentrated through ultrafiltration to prepared the total secreted proteins. Two-dimensional gel electrophoresis (2-DE) was used to separate the secreted proteins of control and experimental cells, PDQuest software was applied to analyze 2-DE images, and the differential protein spots between the control and experimental cells were identified by desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The 2-DE patterns of the secreted proteins of TGF-? stimulated and un-stimulated CNE2 cells were established, 22 differential proteins spots between the two groups of cells were found, and 8 non-redundant proteins were identified with MALDI-TOF-MS, the functions of which were involved in invasion, metastasis, apoptosis and proliferation of cancer cells. The data will be valuable for further to study the role and mechanism of EGFR in the pathogenesis of NPC.
