Expression increase of cyclooxygenase 2 in alcoholic liver disease
- VernacularTitle:环氧合酶-2在酒精性肝病中表达增强
- Author:
Junying ZHOU
;
Shukun YAO
;
Zhen ZHEN
;
Caiyan ZHAO
- Publication Type:Journal Article
- Keywords:
alcoholic liver disease;
cyclooxygenase 2;
oxidative stress
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of cyclooxygenase 2(COX-2) in the pathogenesis of alcoholic liver disease(ALD).Methods A model of ALD was developed with Wistar rats by intragastric alcohol.Liver samples were collected by the end of 4th,8th,12th and 16th week respectively.The concentrations of triglyceride(TG),oxygen free radical(ORF),superoxide dismutase(SOD),anti-superoxide anion(ASOA),6-ketone-prostaglandin F1?(6-K-PGF1?),thromboxane B2(TXB2) and malondiadehyde(MDA) in the liver homogenate were measured by biochemistry chromatometry.Pathology of hepatic tissue was examined by hematoxylin eosin,sudan Ⅳ and sirius red staining.The protein and mRNA expression of COX-2 in liver tissues were examined by immunohistochemical method and reverse transcription-polymerase chain raction(RT-PCR) respectively.Results By the end of 4th,8th,12th and 16th week,the content of COX-2 mRNA in liver tissue of model rats were 1.29,1.70,1.92 and 3.38 times the same as normal rats(P
