Enrichment of dendritic cells precursor from chronic myelogeous leukemia by Percoll density gradients
- VernacularTitle:Percoll密度离心富集慢性粒细胞白血病DC前体细胞
- Author:
Jie LI
;
Mingbai SHAO
;
Lihua KANG
- Publication Type:Journal Article
- Keywords:
Percoll density gradients;
enrichment;
leukemia,myeloid,chronic;
dendritic cells.
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up an effective and low-price way for enriching dendritic cells precursor from chronic myelogeous leukemia by Percoll density gradients.Methods Peripheral blood mononuclear cells(PBMCs) were collected by separation through Ficoll-Hypaque,then PBMCs were separated by 55% Percoll gradients. The cell type and DCs expressing CD1a,CD86 were detected in the high-density group(C),low-density group(B) and non-Percoll separation group(A).Results After separation of 55% Percoll,the percentage of promyelocytes and myelocytes in group B was obviously higher than those in group A and group C(P
