Influence of Danggui buxue tang on the proliferation of bone marrow cells of mice
- VernacularTitle:当归补血汤对骨髓抑制小鼠骨髓细胞增殖的影响
- Author:
Lan YANG
;
Lihua ZHANG
;
Yi ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The experiment was conducted at the Basic Laboratory of Integrated Traditional Chinese and Western Medicine, Chengdu University of TCM from March 2005 to March 2006. A total of 40 BALB/C male mice aged 6-8 weeks were selected, and randomly assigned into 4 groups: bcl-2 control group, bcl-2 experimental group, cmy-c control group and cmy-c experimental group with 10 in each group. From the first day of experiment, all the mice were treated with 5 g/L cyclophosphamide solution by intraperitoneal injection for successively 5 days, 6 mL/kg per day. From days 11 to 12, the mice in the bcl-2 experimental group and cmy-c experimental group received Danggui buxue tang by gastric perfusion, 6 mL/kg per day for 10 days. The mice in the control group received saline of the same volume. At day 20, all the mice were killed to obtain bone marrow cells of one side femoral bone of mice. The smear received bcl-2 in situ hybridization immunohistochemical staining after centrifugalization and washing in the bcl-2 control and bcl-2 experimental group. The smear received cmy-c in situ hybridization immunohistochemical staining after centrifugalization and washing in the cmy-c control and cmy-c experimental group. Five fields of each section were red into image analyzer. The sum of positive cell area and the sum of integral absorbance in each field were analyzed. The results showed that the sum of positive cell area was (16.11?3.01)?102 ?m2, and the sum of integral absorbance was (19.9?2.42)?105 in the bcl-2 experimental group. Compared with the bcl-2 control group, there was significant difference (P
