Detection of Chromosome-encoded ?-Lactamase Gene Families in Klebsiella pneumoniae by Using Nested Polymerase Chain Reaction (nPCR)
- VernacularTitle:套式PCR法检测肺炎克雷伯菌染色体介导?-内酰胺酶基因
- Author:
Jidong WANG
;
Zuhuang MI
;
Xiaomao QIAN
- Publication Type:Journal Article
- Keywords:
Bacterial drug resistance;
Klebsiella pneumoniae;
Chromosome-encoded;
?-Lactamase gene
- From:
Chinese Journal of Nosocomiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To survey the chromosome-encoded ?-lactamase gene families: SHV,LEN and OKP in(continuously) isolated Klebsiella pneumoniae in China.METHODS The existence and statistical distribution of all three gene families in antibiotic resistant K.pneumoniae strains were detected by applying nPCR.RESULTS In 44 strains,28 strains were SHV gene positive(63.6%),4 strains were LEN gene positive(9.1%) and none in OKP gene.CONCLUSIONS All the three gene families are chromosome-encoded and have 80-92% of genetic(homogeneity),so they all could identifiy the most important factors assisting the spread of bacterial drug (resistance).nPCR is an economic and convenient measurement to detect the chromosome-encoded ?-lactamase gene.
