Aminoglycosides Modification Enzymes Genes in Pseudomonas aeruginosa Isolated from ICU Patients
- VernacularTitle:铜绿假单胞菌在重症监护病房分离株氨基糖苷类修饰酶基因研究
- Author:
Huiqin ZHOU
;
Sheng ZHAO
;
Ruhong YAN
;
Yan XIAO
;
Xueming ZHU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Aminoglycosides modification enzymes
- From:
Chinese Journal of Nosocomiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate genes associated with aminoglycosides modification enzymes(AMEs) in Pseudomonas aeruginosa(PAE) isolated from ICU patients.METHODS Drug-reisistant genes encoding AMEs such as aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ were detected by polymerase chain reaction(PCR)(amplification) in 21 PAE isolates.RESULTS The positive rates of aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and aac(3)-Ⅰ genes were positive in 19.0%,23.8%,9.5%,4.8%,19.0% and 0% of 21 isolates,respectively. Drug-resistant genes encoding AMEs were detected positively in 42.8% of 21 isolates.(CONCLUSIONS) AMEs genes are present in high percentage of PAE isolated from ICU patients.
