Purification of AmpC beta-lactamase in Enterobacter cloacae
- VernacularTitle:阴沟肠杆菌AmpC酶的分离纯化
- Author:
Weiyun CUI
;
Yuhua FENG
;
Xiaoxin XU
- Publication Type:Journal Article
- Keywords:
AmpC beta-lactamase;
purification;
capillary electrophoresis
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purify AmpC beta-lactamase from Enterobacter cloacae and study its physical and chemical characteristics.Methods The bacteria were fragmentized by ultrasonication and AmpC beta-lactamase was purified by ion-exchange.The properties of the purified enzyme,such as molecular weight and isoelectric point were determined by electrophoresis.Results The purified AmpC beta-lactamase was obtained and its specificity to substrate was sufficient for classification of class C cephalosporinase.Conclusion AmpC can be successfully purified from Enterobacter cloacae and is effective for application.
