Analysis of correlation factors of intrahepatic hepatitis B virus DNA load in patients with chronic hepatitis B
- VernacularTitle:慢性乙型肝炎患者肝组织中HBV DNA载量相关因素分析
- Author:
Song YANG
;
Gang WANG
;
Guohui MING
;
Lixian MA
;
Lihua SHAO
- Publication Type:Journal Article
- Keywords:
chronic hepatitis B;
peripheral blood mononuclear cell(PBMC);
hepatitis B virus quantitative PCR
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the correlation of intrahepatic HBV DNA load with the HBV load in serum and peripheral blood mononuclear cells(PBMC)and the stage of fibrosis,grade of inflammation,level of serum ALT and AST.Methods Liver specimens were taken from 50 patients by percutaneous needle biopsy and divided into two parts:one was processed for histological examination,and the other was used for molecular biology analyses.HBV DNA load was measured with fluorescent quantitative polymerase chain reaction(FQ-PCR).The data of serum level of ALT and AST were collected.Results A high correlation between intrahepatic HBV-DNA load and serum virus load was found(r=0.77977,P
