Hypoxia enhanced proliferation of C_2C_(12) cells and its possible mechanism
- VernacularTitle:低氧促进C_2C_(12)细胞增殖及相关机制探讨
- Author:
Fan WANG
- Publication Type:Journal Article
- Keywords:
Hypoxia;
C_2C_(12) cells;
Proliferation;
HIF-1?
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of hypoxia in C_2C_(12) cells proliferation.Methods:We chose 10% oxygen concentration.Cell numbers were counted with a hemacytometer.The proliferous indexes were observed with a flow cytometer.HIF-1? protein was determined by Western blot,mRNA expression was determined with reverse transcriptase-polymerase chain reaction(RT-PCR).Results:The cell numbers and proliferous indexes were higher(P
