Dynamic Analysis of TGF-β1 Gene Expressions in Bleomycin-Induced Pulmonary Fibrosis in Rats by RT-PCR
- VernacularTitle:用RT-PCR方法动态分析博莱霉素致肺纤维化大鼠TGF-β1的基因表达
- Author:
Rui ZHENG
;
Jian KANG
;
Xianming HOU
- Publication Type:Journal Article
- From:
Journal of China Medical University
2001;30(2):94-95，100
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Our aim was to evaluate the role of TGF-β1 mRNA in pulmonary fibrosis. Methods: The present study was taken to investigate the expressions of TGF-β1 mRNA in alveolar macrophage（AM) and lung tissues in different stages of bleomycin-induced pulmonary fibrosis in rats through reverse transcript polymerase chain reaction. Results: In bleomycin-treated rats, the expressions of TGF-β1 mRNA was remarkably increased in AM from 3rd day (P< 0.05) , and reached the peak on 7th day (P<0.01) , there was also a continuing high level from 14th day to 28th day (P>0.05). It showed similar pattern with that in lung tissues. Conclusion: There were TGF-β1 mRNA expression in both normal rats and pulmonary fibrosis rats. AMs seemed to be the main source of TGF-β1 mRNA in lung tissues, especially at stage of alveolitis.
