Expression of LTB4-BLT on dendritic cells in mouse
- VernacularTitle:小鼠树突细胞LTB4-BLT的表达
- Author:
Yang YANG
- Publication Type:Journal Article
- Keywords:
leukotriene B4;
leukotriene B4 receptor;
dendritic cell
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate leukotriene B4 secretion from DC and expression of its receptor,BLT(LTB1,LTB2).Methods Bone marrow(BM)-derived DC from healthy Balb/c mice were incubated with rmGS-CSF and rmIL-4,and then stimulated with LPS.Light microscopy and flow cytometry were used to identify DC,ELISA and RT-PCR methods were used to detect LTB4 secretion in culture supernatant and mRNA expression of BLT(BLT1,BLT2) in immaturation and mature DC,respectively.Results LTB4 concentration was(10.667?(2.394)ng/L)、(7.089?(1.810) ng/L)、(3.222?(0.995)ng/L) and(14.217?(3.396)ng/L) in 2,4 and(6 d)(immaturation) and(8 d) (maturation) respectively.LTB4 level decreased progressively during DC differentiation.LTB4 level in mature DC was higher than that in immature DC(P
