The Establishment of ELISA Method for Detecting Babesia gibsoni by Recombinant Antigen BgAMA-1
- VernacularTitle:利用BgAMA-1抗原建立吉布森巴贝斯虫的ELISA诊断方法
- Author:
Jun YANG
;
Jinlin ZHOU
;
Binnan XIAO
- Publication Type:Journal Article
- Keywords:
Babesia gibsoni;
BgAMA-1;
ELISA
- From:
Chinese Journal of Parasitology and Parasitic Diseases
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve diagnostic method to Babesia gibsoni infection,gene recombination technique was used in our study.By this technique,we constructed and expressed recombinant apical membrane antigen-1(BgAMA-1),and recombinant BgAMA-1 was used in ELISA experiment as diagnostic antigen.The results showed the recombinant expressed plasmid was triumphantly constructed and the recombinant antigen BgAMA-1 had a high specificity in ELISA test.These results demonstrate that the recombinant BgAMA-1 might be a useful diagnostic reagent for detection of antibodies to B.gibsoni in dogs.
