Effect of telomerase-antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide
- VernacularTitle:抑制端粒酶活性对As_2O_3诱导肝癌细胞凋亡的影响
- Author:
Zhihua DENG
;
Ziyan HAN
;
Qi WANG
;
Shuisheng SHI
- Publication Type:Journal Article
- Keywords:
telomerase;
antisense oligonucleotide;
As_2O_3 hepatama cell;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of telomerase-antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide, in an effort to look for a new anti-hepatic cancer agent with high efficiency, low cytotoxicity. Methods: We designed and synthesized a 20nt telomerase-antisense DNA targeting telomerase template and observed its influence on the telomerase activity of hepatoma cells. H-E staining, flow cytometry, and DNA agarose electrophoresis were used to study the preventive effect of telomerase-antisense DNA on hepatoma cells apoptosis induced by arsenic trioxide. Flow cytometry was used to detect the expression of Fas, Fas-L, and bcl-2. Results: Telomerase-antisense DNA (5 ?mol/L) effectively inhibited the telomerase activity of hepatoma cells after 24 hours (P
