Study on expression of Cystatin C in gastroenteric tumor
- VernacularTitle:半胱氨酸蛋白酶抑制剂C在胃肠道肿瘤组织中表达的研究
- Author:
Lei WANG
;
Lingyan CHEN
;
Hua LIU
;
Feng GAO
- Publication Type:Journal Article
- Keywords:
Cysteine proteinase inhibitor;
Neoplasms;
Gene expression
- From:
Chinese Journal of Laboratory Medicine
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the contribution of Cystatin C to the preoperative diagnosis and clinical therapy of gastroenteric tumor.Methods Using surgical materials from patients with stomach, colon and rectum cancer, immunohistochemisty, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting analysis were performed with antibodies against Cystatin C.Cystatin C of the cancerous and paired noncancerous lesions were determined by enzyme-linked immunosorbent assay (ELISA).Results (1)Immunohistochemical staining of Cystatin C was evident expression in cancer cells and associated stromal tissues, this was not the case in paired noncancerous tissues,there were also statistical relationship between the expression of Cystatin C in cancerous and that in noncancerous tissues (?~2=6.825,P
