Study of mitochondrion and nucleus damage and caspase-3 expression in hippocampal CA_3 neurons of kainic acid induced epileptic rats
- VernacularTitle:海人酸致癎大鼠海马CA_3区神经元线粒体与细胞核损伤及caspase-3表达的研究
- Author:
Jianying SUN
;
Zhaofu CHI
;
Wei WU
- Publication Type:Journal Article
- Keywords:
status epilepticus;
mitochondrion;
nucleus;
caspase-3
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mitochondrion and nucleus ultrastructural damage and caspase-3 expression in hippocampal CA_3 neurons during kainic acid(KA) induced status epilepticus(SE) in rats.Methods SE was induced for 2 h with KA in adult male Wistar rats.3,12 and 24 h later the rats were killed and the hippocampal CA_3 subareas were taken out to make brain sections.The neuronal damage on the whole with light microscope and the ultrastructure of mitochondrion and nucleus with electron microscope.Caspase-3 expression of the same area was examined with immunohistochemical staining.Results 24 h after SE,by the light microscope examination,the KA group showed that the neurons put scattered disorder and nucleus shrink firmly.3 h after SE,electron microscope examination showed swelling cristae and ruptured membrane of mitochondria.The change of nucleus were significant margination of chromatin 24 h after SE.Compared to normal control group,the caspase-3 expression increased 12 h after SE,the average number of positive cell and the gray scale were obviously higher(all(P
