Expressions of neurogranin in rat cortex and hippocampus after kainic acid induced seizures
- VernacularTitle:神经颗粒素在海人酸诱发癫癎大鼠皮质及海马中表达的研究
- Author:
Guohong TIAN
;
Ming FANG
;
Liuqing HUANG
- Publication Type:Journal Article
- Keywords:
epilepsy;
neurogranin;
kainic acid;
synaptic plasticity
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neurogranin expression in the rat neocortical and hippocampal regions after kainci acid(KA) induced limbic seizures.Methods 52 SD rats were divided randomly into KA group and normal control group.The animals of KA group were injucted KA to kindling the seizures and sacrificed at the end of 6 h,12 h,18 h,24 h,and 48 h respectively after seizures induced.By using fluorescent immunostain combined with confocal microscope,neurogranin expression and distribution were examined in the cerebral cortex and different regions of hippocampal.Western Blot technique was specially used to analyze the quantitative level of protein involved in the relate areas.Results Strong expression of neurogranin was present in cytoplasm of layers Ⅱand Ⅲ cortical,CA_1,CA_3 pyramidal cells and dentate gyrus granule cells of normal control groups.Rats after 18 h KA injection began to exhibit decreased expression of neurogranin in the cortex significantly((P
