Cloning of L-Methionine ?-lyase gene
- VernacularTitle:蛋氨酸裂解酶的基因克隆及序列分析
- Author:
Baikun MA
- Publication Type:Journal Article
- Keywords:
L-Methionine ?-lyase;
Gene cloning;
Reverse transcription PCR
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify,clone,sequence the L-Methionine ?-lyase gene from Trichomonas vaginalis(TVMGL1).Methods:Total RNA was prepared from primitive protozoon trichomonas vaginalis,cDNA fragment encoding Methionine ?-lyase gene was amplified by RT-PCR using specific primers,and then was cloned into pGEM-T vector.Inserted Methionine ?-lyase gene was sequenced by ABI 3730 DNA Sequencer.Results:Analysis indicates that the DNA fragment is 1 191 base pairs in length and has high nucleotide homology with that reported previously.Conclusion:L-Methionine ?-lyase gene was successfully cloned.
