Inhibitory effects of rosiglitazone on activation of NF-?B and expression of ICAM-1 induced by high glucose in rat mesangial cells
- VernacularTitle:罗格列酮对高糖诱导的系膜细胞核因子-?B活性及细胞间黏附分子1表达的影响
- Author:
Hui LI
;
Dajin ZOU
;
Yitao JIA
- Publication Type:Journal Article
- Keywords:
Rosiglitazone;
Nuclear factor-?B;
Intercellular adhesion molecule-1;
Mesangial cells
- From:Chinese Journal of Diabetes
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of rosiglitazone in high glucose(HG)-induced intercellular adhesion molecule-1(ICAM-1) expression of rat mesangial cells.Methods The rat mesangial cells(MCs) were cultured in the medium with normal glucose(5.6 mmol/L,NG),high glucose(25 mmol/L,HG),HG+5 ?mol/L rosiglitazone,HG+20 ?mol/L rosiglitazone and HG+PDTC(?NF-?B inhibitor).ICAM-1 mRNA expression was measured by semi-quantitative RT-PCR assay.Activation of nuclear factor-?B(NF-?B) of rat mesangial cells was measured by electrophoretic mobility shift assay(EMSA).The levels of ICAM-1 in the supernatants were determined by enzyme-linked immunosorbant assay(ELISA.) Results RT-PCR results showed that high-glucose increased the ratio of PCR products of ICAM-1 over GAPDH to 2.9-fold,which was prevented by rosiglitazone(5 and 20 ?mol/L) pre-treatment.The NF-?B binding activity was 2.5-fold higher in MCs exposed to HG as compared with NG(P
