Comparison of QRDR Mutations in Fluoroquinolone-resistant Klebsiella pneumoniae Between Fluoroquinolone Inducing Strains in vitro and Clinical Isolates
- VernacularTitle:体外氟喹诺酮类诱导肺炎克雷伯菌耐药与临床分离耐药株QRDR变异的差异研究
- Author:
Xiaoguang YE
;
Jumei HAN
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Fluoroquinolone;
Induction;
Mechanism of drug-resistance;
DNA gyrase;
QRDR
- From:
Chinese Journal of Nosocomiology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the differences of quinolones-resistant determining region(QRDR) in DNA gyrase A(gyrA) subunit between drug-resistant Klebsiella pneumoniae induced by fluoroquinolone in vitro and strains isolated from clinical isolates.Furthermore,to reveal the relation between different QRDR gene mutations and drug-resistance. METHODS Ten sensitive K.pneumoniae strains were selected and induced into drug-resistant strains by ciprofloxacin.The QRDR in gyrA by polymerase chain reaction(PCR) was amplified and compared their DNA sequences with clinically isolated quinolone-resistant K.pneumoniae. RESULTS We had found several gene mutations of QRDR in fluoroquinolone-resistant K.pneumoniae induced by ciprofloxacin,including Ser 83(TCC)→Phe(TTC) and Ile(ATC);and Gln106(CAG)→Leu(CTG).And the alteration of Gln106(CAG)→Leu(CTG) was a new discovery. CONCLUSIONS There are gene mutations of QRDR in fluoroquinolone-resistant K.pneumoniae induced by ciprofloxacin.The mutation of Ser 83(TCC) and Gln106(CAG) is probably the molecular basis of inducing drug-resistant strains.
