Gene Polymorphisms of Imipenem-resistant Pseudomonas aeruginosa
- VernacularTitle:耐亚胺培南铜绿假单胞菌的基因多态性研究
- Author:
Sujian WANG
;
Jiaping WANG
- Publication Type:Journal Article
- Keywords:
Imipenem;
Gene polymorphisms;
Pseudomonas aeruginosa
- From:
Chinese Journal of Nosocomiology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the genetic polymorphism of imipenem-resistant Pseudomonas aeruginosa by randomly amplified polymorphic DNA(RAPD). METHODS The genes of imipenem-resistant P.aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis. RESULTS The phenotypes of drug resistance were the same,but most of the genotypes were different,even though some of them could be equal. CONCLUSIONS RAPD assay reveals the special genotypes of imipenem-resistant P.aeruginosa and makes the relation between the phenotype of drug resistance with genotype of drug resistance definite,which provides molecular epidemiological evidence for strategies to control resistant imipenem P.aeruginosa infections.
