Regulatory effects of hippocampus extracts on PI4-K activity
- VernacularTitle:海马提取物调控PI4-K激酶活性
- Author:
Fang CHEN
- Publication Type:Journal Article
- Keywords:
phosphtidylinositol 4-Kinase,extracts of hippocampus,indirect enzyme-linked immunosorbent assay,inside-out vesicle,medicine screening
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
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Abstract:
Objective The hippocampus has been used in Chinese medicine for a long time.Although it has the anti-decrepitude,anti-fatigue and anti-tumor effect,the molecular mechanism is not yet known.PI4-K,the first protein kinase in the PI pathway,should be the target molecular for drug screening.Methods We prepared antigen protein IOV-PI4-K through inside-out ghost,then the antibody-antigen reaction between IOV-PI4-K and its antibody A6D was tested by ELISA.Results Two different function groups,which could increase or decrease the binding affinity of PI4-K to its antibody,were screened out from the extracts of hippocampus.This fact indicated that there might be two different components to up-regulate or down-regulate the activity of PI4-K respectively.Conclusion Derivatives of the components could be developed into anti-tumor or anti-decrepitude drugs.Meanwhile,a new way for performing drug screen through ELISA was established.
