Effect of mesenchymal stem cell on the rate of Th1/Th2 with different stimulations
- VernacularTitle:间充质干细胞对不同刺激条件下Th1/Th2细胞比值的影响
- Author:
Min XUAN
;
Xiaobao XIE
;
Guoqiang QIU
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
phorbol ester;
mixed lymphocyte culture;
cytokine
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mesenchymal stem cell(MSC) on the balance of Th1/ Th2 with different stimulations.Methods To inoculate MSC in 24-well tissue culture plates.after 3 days, added T lymphocytes co-stimulated by PMA and Ionomycin,or the MSC were added to the two-way mixed lymphocyte culture according to different proportion.The subsets and cytokines of T lymphocytes were analyzed by flow cytomety.Results Differentiation into Th1 of T lymphocytes activated by co-stimulation can be inhibited MSC;In MLC,CD8~+T cell subsets and Th1 were evidently decreased.But Th2 cells were slightly increased.Conclusion MSC can significantly suppress CD8~(+)T cell,Th1,increase Th2.MSC has potentialities of alleviating acute graft-versus-host disease(aGVHD) and maintaining graft versus leukemia(GVL).
