Flaer detection for paroxysmal nocturnal hemoglobinuria
- VernacularTitle:阵发性睡眠性血红蛋白尿症的嗜水气单胞菌溶素变异体检测
- Author:
Bing HAN
;
Xuan WANG
;
Daobin ZHOU
;
Yongqiang ZHAO
- Publication Type:Journal Article
- Keywords:
paroxysmal bocturnal hemoglobinuria(PNH);
Flaer;
flowcytometer
- From:
Basic & Clinical Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new method using Flaer for detecting abnormal clone in patients with paroxysmal nocturnal hemoglobinuria(PNH).Methods Peripheral WBC and bone marrow mononuclear cells from patients with PNH and normal controls were isolated and stained with flaer,CD55 PE,CD59 FITC and CD34 PE.Results PNH cells can easily be distinguished by flare.Compared with CD55,CD59,Flaer showed advantage in detecting minor clone of PNH,either in peripheral blood or in CD34+ bone marrow cells.Conclusion Flaer can be a new,simple and effective method to detect PNH clone,and especially when the PNH clone is small.
