Anti-tumor effect of IL-12 combined with B7-1 gene radiotherapy on mice transplanted with B16 melanoma
- VernacularTitle:IL-12联合B7-1基因放射治疗对小鼠B16移植肿瘤生长的影响
- Author:
Ying YANG
;
Shibo FU
;
Shuzheng LIU
- Publication Type:Journal Article
- Keywords:
interleukin-12;
antigens,CD80;
radiation,ionizing gene therapy;
neoplasms,experimental
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effects of gene combined radiotherapy on mice transplanted with B16 melanoma.Methods Alkaline lysis assay was used to extract and purify the plasmid.Plasmid DNA was injected into tumor by microinjection assay.Mice were inoculated with 5?10~5 of B16 melanoma in right hind legs and the therapy was performed when the diameter of tumor reached at 0.30.5 cm.pNE-mIL-12 and pcDNA-B7-1 plasmids were injected locally three times following with irradiation three times.The tumor growth rate of mice was observed.Results The anti-tumor effect of pNE-mIL-12 combined with pcDNA-B7-1 plasmid following with 2 Gy X-ray irradiation was much better than other groups.It showed that the tumor growth rate was slowed,the survival days of mice were delayed significantly(P
