Effects of fluoride on expressions of TGF-? and Smad2/3 in fibroblasts and osteoblasts
- VernacularTitle:氟对成纤维细胞和成骨细胞TGF-?和Smad2/3表达的影响
- Author:
Yizhuo ZHAO
;
Ling QI
;
Hui XU
;
Ling JING
- Publication Type:Journal Article
- Keywords:
transforming growth factor beta;
fibroblasts;
osteoblasts;
enzyme-linked immunosorbent assay;
reverse transcriptase polymerase chain reaction
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of fluoride with different doses on the expressions of TGF-? and Smad2/3 in fibroblast and osteoblast at different periods.Methods Fibroblasts and osteoblasts were exposed to different concentrations of fluoride(0,0.0001,0.001,0.1,1,10 and 20 mg?L~(-1)).The levels of TGF-? protein and Smad2/3 at 2,4,24,48 and 72 h after treatment were measured by using ELISA method.The expression of TGF-? mRNA was tested with RT-PCR method.Results In fibroblasts,the contents of TGF-? protein were decreased in the groups of 0.001,0.1,1,10 and 20 mg?L~(-1) F~-at the time of 2 h and in the groups of 1.0001,0.001,0.1,10 and 20 mg?L~(-1) at the time of 4 h(P
