Effects of recombinant murine interlukin-12 and GM-CSF gene adenovirus vectors on B16 melanoma
- VernacularTitle:重组鼠白细胞介素12与粒细胞-巨噬细胞集落刺激因子基因联合治疗小鼠B16黑色素瘤
- Author:
Hang GAO
;
Jianwei WANG
- Publication Type:Journal Article
- Keywords:
Interleukin-12;
granulocyte-macrophage colony-stimulating factor;
melanoma;
experimental;
gene therapy
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-tumor effects of combined recombinant murine interleukin-12 and GM-CSF gene adenovirus vector(AdCMVIL-12,AdCMVGMCSF) on B16 melanoma.Methods B16 melanoma cells were injected subcutaneous at right back of C57/BL6 mice,48 tumor bearing mice were divided into 4 groups at random,when tumor diameter attained to 5 mm,AdCMVIL-12,AdCMVGM-CSF and AdCMVIL-12+AdCMVGM-CSF and AdCMVLacZ(control) were injected in different groups,tumor growth and immune responses of each group were tested,and the IL-12 and GMCSF levels in serum were observed.Results In combined group,necrosis, lymphocytes and macrophages were found in tumor tissues.30 d after treatment,the average volume in combined group was(60.73?11.36)mm~3, compared with AdCMVLacZ(control) group,and AdCMVIL-12 group,AdCMVGM-CSF group [(675.18?80.59),(186.91?19.15),(223.45?23.11)mm~3],there were significant differences(P
