Relationship between CXXC motif and biologic activity of hALR
- VernacularTitle:人肝再生增强因子CXXC结构域与生物学活性的关系
- Author:
Yan PAN
;
Guizhi JU
- Publication Type:Journal Article
- Keywords:
human augmenter of liver regeneration;
mutagenesis;
GST-pulldown assay
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between CXXC motif and biologic activity of human augmenter of liver regeneration protein(hALRp).Methods The function of CXXC motif and its relationship with bioactivity were investigated between hALRp and hALRp-C65A groups by detecting the sulfhydryl oxidase activity;the interaction of target protein with GST-Na~+,K~+-ATPase fusion protein was examined by GST-Pulldown and MTT was used to study the ability of hALRp-C65A to promote HL-7702 hepatic cells proliferation in vitro.Results The turnover number(TN) of hALRp in sulfhydryl oxidase activity assay was 1.25?0.21,while TN was 0 of hALRp-C65A,there was significant difference between them(P
