Genotypes of hepatitis B virus in Changchun
- VernacularTitle:长春地区乙型肝炎病毒基因分型
- Author:
Lingling ZHANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus/classification;
genotype;
sequence analysis
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotypes of hepatitis B virus in chronic liver disease patients in Changchun,China.Methods HBV DNA extracted from the 39 serum samples of chronic liver disease patients were amplified by nested polymerase chain reaction(nested-PCR) to obtain the S gene sequences,and all the PCR products were sequenced by dideoxy-chain-termination method.Results The S gene nucleotide sequence was acquired and the corresponding amino acid sequence was deduced.Compared with the consensus sequence,(16 strains) belonged to genotype C/adr and 7 strains to genotype C/adw;16 strains belonged to genotype B/adw;and the amino acids of antigen determinant "a" of two strains changed in the 2 serum samples.There were many mutational positions and some of these caused amino acid residue substitution.No special point mutation was found which was similarly related to hepatocellular carcinoma.Conclusion In chronic liver diseases patients infected with HBV,S gene sequence of HBV has its variability.
