Mechanism of change in runx3 in gastric carcinoma and its difference with p53 mutation
- VernacularTitle:胃癌组织中Runx3的变化机制及其与p53突变的对比
- Author:
Hong XU
;
Peng BIAN
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
runx3;
gene p53;
DNA methylation;
mutation
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mechanism of the change in runx3 in gastric carcinoma its difference and with p53 mutation.Methods A total of 30 cases of gastric carcinoma were taken from clinical operation.PCR-SSCP method was used to detect mutations in exons 2,3,4 of runx3 gene in the gastric carcinoma.The methylation status of runx3 gene was examined by methylation-specific polymerase chain reaction(MS-PCR),PCR-SSCP method was used to detect mutations in exons 5,6,7,8 of p53 gene in the gastric carcinoma.Results Two cases with mutation were found,methylation of runx3 promoter region was confirmed in 87%(26/30) specimens of gastric carcinoma,53.3%(16/30)cases were confirmed with mutation in the p53 gene.The rate of methylation of runx3 was higher than the rate of mutation of p53(P
