The study on the time resolved fluoroimmunoassay of double labeled prostate specific antigen
- VernacularTitle:双标记前列腺特异性抗原时间分辨荧光免疫分析试剂盒研究
- Author:
Lan ZHU
;
Biao HUANG
;
Xiangrui ZHANG
- Publication Type:Journal Article
- Keywords:
Prostate specific antigen;
Prostatic neoplasms;
fluoroimmunoassay
- From:
Chinese Journal of Laboratory Medicine
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the time-resolved fluoroimmunoassay (TRFIA) of total prostate specific antigen (tPSA) labeled with Sm 3+ and free prostate specific antigen (fPSA) labeled with Eu 3+ . Methods The monoclonal antibody (McAb) 101# was coated on the 96 well plates, anti-tPSA McAb 102# was labeled with Sm 3+ and anti-fPSA McAb 201# was labeled with Eu 3+ .The standard curves were given out by Log-Log_B function of the DEFIA instrument.The levels of PSA in sera from patients or healthy volunteers were determined by t/fPSA TRFIA using the autoDELFIA_ 1235 system.Results The measurement ranges of tPSA-TRFIA were 0.02-250 ?g/L and those of fPSA-TRFIA were 0.05-250 ?g/L. The within-run and between-run CVs of the tPSA-TRFIA were 2.38% and 3.91%, respectively, and those of fPSA-TRFIA were 3.16% and 3.34%, respectively. The recovery rates of tPSA-TRFIA and fPSA-TRFIA were 101.3% and 103.2%, respectively.The detection limitations of tPSA and fPSA were 0.05 ?g/L and 0.02 ?g/L,respectively.The average results of health group benign prostate disease patients,prostate cancer patients for tPSA were 1.4 ?g/L,4.3 ?g/L,14.2 ?g/L,and those of fPSA were 0.3 ?g/L,1.5 ?g/L,6.2 ?g/L.The cut-off point condition for benign prostate disease and malignant prostate cancer was tPSA
