Calcineurin mediates the collagen synthesis of rat cardiac fibroblasts induced by arginine vasopressin
- VernacularTitle:钙调神经磷酸酶调控血管升压素诱导大鼠心脏成纤维细胞胶原合成的作用
- Author:
Fujun SHANG
- Publication Type:Journal Article
- Keywords:
Arginine vasopressin;
Calcineurin;
Ciclosporin;
Cardiac fibroblasts;
Collagen synthesis
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the effects of calcineurin signaling pathway on the collagen synthesis of rat cardiac fibroblasts(CF) induced by arginine vasopressin(AVP). Methods:CF of neonatal Sprague-Dawley rats were secured by trypsinization and Cultured.Collagen content of CF culture medium was estimated by hydroxyproline colorimetry.Calcineurin activity was determined with spectrophotometer.Results:① AVP increased the content of hydroxyproline in culture medium of CF in a concentration dependent manner.But in the presence of 0.5 ?g/ml cyclosporin A(CsA),the specific inhibitor of calcineurin,the hydroxyproline content of culture medium in corresponding AVP+CsA group was significantly lower than that of 10~(-7) mol/L AVP or 10~(-6)mol/L AVP group(both P
