Study on alpha-fetoprotein chemiluminescence immunoassay by microfluidic chip
- VernacularTitle:微流控芯片用于甲胎蛋白化学发光免疫分析的研究
- Author:
Hui HUANG
- Publication Type:Journal Article
- Keywords:
Superparamagnetic microbeads;
Chemiluminescent;
Microfluidic chip;
Immunoassay
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a microfluidic chip system for immunoassay.Methods:We use direct-writing laser micromachining technology to fabricate microfluidic chip,establish microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads,and use this system to detect alpha-fetoprotein. Results:We can complete the analysis within 20 minutes with 5?l sample or reagent,the linear range was 1-800 ng/ml with average intra-assay and inter-assay precision at 6.3% and 7.8%,respectively and average recovery at 94.3%. Conclusion:The microfluidic chip system is a time-saving,cost-low and convenient method for AFP analysis.
