Piezoelectric Gene Biosensors for Detection of Pseudomonas aeruginosa Using Signal Amplification Method with Cationic Conjugated Polymers
- VernacularTitle:阳离子多聚体信号放大方法应用于压电基因生物传感器检测铜绿假单胞菌的研究
- Author:
Feng WANG
;
Weiling FU
;
Xueqing XU
;
Han XIA
;
Yang LUO
- Publication Type:Journal Article
- Keywords:
Piezoelectric sensor;
Pseudomonas aeruginosa;
Cationic conjugated polymers;
Signal amplification
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To discuss the feasibility of signal amplification method with cationic conjugated polymers(liposome) applied during the detection of Pseudomonas aeruginosa using piezoelectric gene biosensors.(METHODS) Oligonucleotide probe for P.aeruginosa was immobilized on the surface of gene sensor array and(hybridized) by PCR production of P.aeruginosa.After hybridization,liposome was added.The frequency shifts were recorded and compared with those ones of the control groups.RESULTS The frequency shifts were(significantly) increasing when adding liposome and the compatible concentration of liposome was 0.8?g/?l.(CONCLUSIONS) Liposome signal amplification is proved to be an effective method to amplify the piezoelectric(signal).
