Immobilization Method on Piezoelectric Quartz Crystal DNA Sensors Based on Monolayer SAM
- VernacularTitle:基于自组装单分子层技术的压电石英晶体DNA传感器固定方法的研究
- Author:
Han XIA
;
Weiling FU
;
Ming CHEN
;
Feng WANG
;
Yuhui ZHAO
- Publication Type:Journal Article
- Keywords:
Quarz crystal microbalance(QCM);
Probe;
Self-asembly method(SAM);
Pseudomonas aeruginosa
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To discuss a highly effective method to immobilize probe on the surfaces of piezoelectric DNA sensors.METHODS Pseudomonas aeruginosa probe was immobilized on the gold surface of gene sensor(array) with routine self-assembly method(SAM)(non-reduction method) and SAM with deoxidized probe((reduction) method),respectively.The changes in frequency and time-cost were compared in reactions with(different) concentrations of probe.RESULTS Reduction method had the advantage of more probe immobilization;less time consumed in testing and higher changes in frequency during the reaction than non-reduction method.CONCLUSIONS Reduction method has a better ability to immobilize probe on the surfaces of piezoelectric DNA sensors.
