Identification of Clostridium perfringens Causing Human Disease by Multiplex PCR
- VernacularTitle:应用多重PCR鉴定对人致病的产气荚膜梭菌毒素
- Author:
Yuhui ZHAO
;
Weiling FU
;
Ming CHEN
;
Qing HUANG
;
Han XIA
- Publication Type:Journal Article
- Keywords:
Clostridium perfringens;
Toxin;
Gene;
Multiplex PCR
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To search a method for identifying Clostridium perfringens and genotyping their toxin for gene diagnosis by multiplex PCR.METHODS The mutiplex PCR was developed with three sets of primers(designed) based on the sequences of three C.perfringens toxin genes(CP?,CP? and CPE) published in GenBank to identify C.perfringens and genotype their three toxin genes.RESULTS Three expected(sequences) were (obtained) successfully by multiplex PCR and identified by electrophoresis.CONCLUSIONS The(specific) sequences of C.perfringens could be amplified and their three genes of toxins could be identified by this multiplex PCR(system).Such method should be helpful for developing gene diagnosis well.
