DNA Staining Efficiencies of Different Fluorescent Dyes in Preparative Agarose Gel Electrophoresis:A Comparative Analysis
- VernacularTitle:核酸荧光染料在琼脂糖凝胶电泳中的染色特性
- Author:
Qing HUANG
;
Weiling FU
;
Yuhui ZHAO
;
Han XIA
;
Junfu HUANG
;
Bin CHEN
- Publication Type:Journal Article
- Keywords:
Nucleic acid staining;
Ethidium bromide;
Fluorescent dyes
- From:
Chinese Journal of Nosocomiology
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To set-up safe alternatives to ethidium bromide(EB).METHODS Comparative analysis was performed on the DNA staining efficiencies of 4 fluorescent dyes including SYBR Gold,SYBR Green Ⅰ,(GoldView) and EB in preparative agarose gel electrophoresis.RESULTS Although both SYBR Gold and SYBR Green Ⅰ altered electrophoretic mobility and thus DNA size estimates,they were cost-effective alternatives to EB.SYBR Gold was more sensitive than SYBR Green Ⅰ at detecting short fragments,but 50 bp bands were clearly(visible) using either dye when visualized with a long integration time.CONCLUSIONS SYBR Gold or SYBR Green Ⅰ are sensitive and relatively safe alternatives to EB.
