Association between atopy for Platanus Acerifolia pollen and HLA-DRB1 alleles
- VernacularTitle:二球悬铃木花粉变应原特应性体质与HLA-DRB1等位基因相关性分析
- Author:
Ming QI
;
Hua WEI
;
Qin ZHU
;
Aili WANG
;
Hua XIONG
- Publication Type:Journal Article
- Keywords:
HLA;
allele;
Platanus Acerifolia pollen;
allergen;
PCR-SSP(polymerase chain reaction-sequence specific primer)
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a PCR-SSP method for detection of HLA-DRB1 alleles in the patients who were hypersensitive to Platanus Acerifolia pollen allergen,and to probe into the association between the atopic subjects to Platanus Acerifolia pollen allergen and HLA-DRB1 alleles.Methods DNA in whole blood was extracted by phenol-chloroform method.Eight pairs of specific primers for alleles were synthesized,and HLA-DRB1*0401,*0402,*0403,*0404,*0405,*0406,*0407,*0408 alleles in 20 atopic patients and 36 healthy individuals of Jiangsu Province with Han nationality were detected by PCR-SSP(polymerase chain reaction-sequence specific primer).Results By optimizing the experimental conditions PCR-SSP methods for detection of the 8 alleles were established and the distributing data of above-mentioned HLA DRB1 were obtained.The frequency of HLA DRB1*0405 and *0406 in the patients group was higher than that of in healthy controls group,while the frequency of HLA DRB1*0402 in the patients group was lower than that in controls.No significant deference for the other 5 alleles was found between the 2 groups.Conclusion HLA-DRB1*0406和*0405 seems to be the likely suspected candidate alleles responsible for susceptibility to Platanus Acerifolia pollen allergen in the atopic patients,while DRB1*0402 might be contribute to the related resistance to the allergen.
