Histological research of calcium phosphate biomaterials promoting tendon healing in bone tunnels
- VernacularTitle:采用骨诱导性钙磷生物材料促进肌腱在骨隧道内愈合的组织学研究
- Author:
Hao SHEN
;
Hongbin CAO
;
Yao JIANG
- Publication Type:Journal Article
- Keywords:
Tendon;
Bone tunnel;
Calcium phosphate biomaterial;
Histology
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of osteoinductive calcium phosphate ceramics (OICPC) on tendon healing in bone tunnels. Methods Anterior cruciate ligament reconstruction with semitendonous tendon was performed in the knee joints of 30 New Zealand rabbits by suspended fixation. OICPC grains were implanted into the bone tunnel of 15 rabbits which were the experimental group and the other 15 rabbits without OICPC implants severed as the control group. Specimens were collected four weeks, eight weeks and 12 weeks after surgery. Sections were stained with H. E., Sirius Red and Masson. Histological changes in the interface tissue between the bone tunnel and the tendon graft were observed, and the morphology of interface tissue was classified according to Yamakado method. Histomorphometry was done on Masson stain sections to measure new bone formation around the grafted tendon. Results In OICPC group, histological sections obtained four and eight weeks after surgery showed statistically greater new bone formation than those in the control group. Abundant penetrating Sharpey fibers appeared at eight weeks and direct connection was found at 12 weeks in the experimental group. On the other hand, Sharpey fibers appeared only at 12 weeks in the control group. Conclusion OICPC has a capability of enhancing biological fixation of a tendon to the bone tunnel.
