The gene expressions involved in osteogenic differentiation of bone marrow stromal cells of mice in vitro
- VernacularTitle:体外诱导小鼠骨髓基质干细胞向成骨细胞分化相关基因表达的研究
- Author:
Lin WANG
;
Deyu LIU
;
Xinquan SHI
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells (BMSCs);
Gene expression;
Real-time RT-PCR
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To qualitatively analyze the expressions of key marker genes involved in osteogenic differentiation of bone marrow stromal cells (BMSCs) of mice at defined stages in vitro using real-time RT-PCR. Methods The BMSCs of mice were cultured in vitro and induced to osteoblasts for 7, 14, and 21 days. The total RNA was extracted from the cultured cells and the gene expression levels of collagenⅠ, bone alkaline phosphatase (APL), osteopontin (OPN) and bone sialoprotein (BSP) in the osteoblasts at the defined stages were determined by real-time RT-PCR. Results The ColⅠmRNA levels in 14 and 21 days were (0. 75?0. 04) and (0. 34?0. 03) times respectively of that in 7 days. ALP, OPN and BSP mRNA levels in 21 days were (19. 70?2. 36), (150. 12?9. 31) and (7. 73?0. 58) times of those in 7 days respectively. Conclusions ColⅠexpression levels tend to gradually decline while ALP, OPN and BSP levels gradually increase along with the culture time. Real-time RT-PCR is a reliable method for investigation of gene expressions in BMSCs cultured in vitro.
