siRNA inhibits HPV18 E6 gene and its effect on the apoptosis of HeLa cells
- VernacularTitle:siRNA抑制HPV18E6基因及其对HeLa细胞凋亡的影响
- Author:
Min WEI
;
Wenli MA
- Publication Type:Journal Article
- Keywords:
siRNA;
HeLa cells;
HPV18 E6 gene;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of small interfering RNA(siRNA) of human papillomavirus(HPV) 18(E6) gene on apoptosis of HPV-related cervical HeLa cell line.Methods siRNA targeting HPV18 E6 mRNA was designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA sequence were then transfected into HeLa cells via Lipofectamine()~(TM)2000.Cell viability was determined by MTT assay.Apoptosis was detected by morphological observation and flow cytometry analysis.The expression level of HPV18 E6 mRNA was assayed by RT-PCR.Results The cell growth and viability of(siRNA) transfected group were significantly inhibited(P
