Vascular calcification model of bovine aortic smooth muscle cells in vitro
- VernacularTitle:牛主动脉平滑肌细胞体外钙化模型的制备
- Author:
Lixin LIU
- Publication Type:Journal Article
- Keywords:
calcification;
aorta;
smooth muscle cell;
-glycerophosphate;
in vitro model
- From:
Basic & Clinical Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an in vitro vascular calcification model of bovine aortic smooth muscle cell(BASMC) induced by ?-glycerophosphate.Methods: Primary BASMCs were obtained by the explant method.The cells after 4～8 passages were incubated in the medium containing(10 mmol/L) ?-glycerophosphate for 10 days to induce calcification.Calcification were observed in cultures by Alizarin Red S and Von Kossa stain and transmission electron microscopy.Meanwhile,calcium deposition,alkaline phosphatase(ALP) activity of cell layer and osteocalcin(OC) in culture supernatant were measured.Results After 10 days,extensive calcium deposition was detected by Von Kossa and alizarin red S stain and transmission electron microscopy.?-glycerophosphate increased calcium deposition in a time-dependent manner,ALP activity and OC concentration were higher than that of uncalcified controls at various time points(P
