Separation of HSP70-peptide compound and the expression of HSP70 in esophagus cancer tissue
- VernacularTitle:食管癌组织中HSP70的基因表达及其多肽复合物的分离
- Author:
Anfang ZHAO
- Publication Type:Journal Article
- Keywords:
expression of HSP70;
northern hybrid;
HSP70-peptide compound;
esophagus cancer
- From:
Basic & Clinical Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim is to expore the expression of HSP70 in human esophagus cancer and introduce method of separation of HSP70-peptide compound.Methods The expression of human HSP70 RNA in human(esophagus) cancer was hybridized with P17 probe by Northern blot.The HSP70-peptide complex was isolated by(affinity) chromatography.Results HSP70 mRNA was detected in human esophagus cancer tissue at much more higher level than corresponding normal tissue;HSP70-peptide compound are separated from esophagus cancer of human body.Conclusion This research not only testified the strong expression of HSP70 in esophagus cancer,but also supplied a practical method of extraction and purification of HSP70-peptide compound of tumor origin.This study makes a starting point for further research in this field.
