Wnt3a is Important in The Differentiation From Neural Stem Cell Into Dopaminergic Neuron In vitro
- VernacularTitle:Wnt 3a在神经干细胞向多巴胺能神经元分化过程中作用的研究
- Author:
Shu HAN
;
Wei SHI
;
Yanhua LI
;
Hailei YAO
;
Xiaoyan XIE
;
Lin CHEN
;
Shuangshuang SHI
;
Cixian BAI
;
Xue NAN
;
Fang YAN
;
Yunfang WANG
;
Xuetao PEI
- Publication Type:Journal Article
- Keywords:
Wnt3a, neural stem cell, differentiation, dopaminergic (DA) neuron
- From:
Progress in Biochemistry and Biophysics
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Wnt signaling is implicated in the control of cell growth and differentiation during neural stem cell(CNS) development.Wnt3a, one of wnt gene family members, has effect on regeneration neurospheres and differentiation into neurons.Wnt3a inhibits regeneration of neurospheres, and promotes its differentiation. In vitro neurosphere was cultured in a serum-free defined medium DMEM/F12 supplemented with bFGF and EGF. Dissociated cells were plated onto poly-d-lysine-coated coverslips and propagated in medium containing recombined Wnt3a-adenovirus. Plenty of Nurr1 were detected by RT-PCR after 3 days. Wnt3a combined AA would improve NSC differentiation into dopaminergic (DA) neuron. The quantity of DA neuron is obviously more than the AA alone group's. Moreover, the expression of TH mRNA is 1.86 fold in Wnt3a combined AA group. Induced cells were immunostained for TH and DAT. The proportion of TH-positive was (37.42 ? 2.54) % (P
