Studies on the Immuno-Protection of ROP2 Nuclei Acid Vaccine in Toxoplasma gondii Infection
- VernacularTitle:弓形虫ROP2核酸疫苗免疫保护作用的研究
- Author:
Qingkuan WEI
;
Jin LI
;
Tingxia FU
;
Xuelian BAI
;
Yong CUI
;
Dianbo ZHANG
;
Hongfa WANG
;
Yubing LIU
;
Bin FU
;
Defu ZAI
;
Bingcheng HUANG
;
Keyi LIU
;
Guangdong HAN
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Rhoptry protein(ROP2);
Nuclei acid vaccine;
Immuno-protection
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of ROP2 nuclei acid vaccine in mice.Methods Forty-two BALB/c mice were divided into three groups.Each mouse in experiment group was injected with 50 ?g recombinant plasmid pc-DNA3-ROP2 through musculus quadriceps fexoris.In control groups,each mouse was injected with 50 ?g blank plasmid pc-DNA3 and with 50 ?l PBS respectively.All mice were immunized for three times with an interval of three weeks.The volume was doubled for the final injection in the two plasmid groups.Blood,spleens and lymph nodes of 4 mice in each group were taken for the detection of CD4+,CD8+ T cells and cytokines 2 weeks after the final immunization.The rest mice in 3 groups were challenged with 500 tachyzoites of Toxoplasm gondii RH strain for further observation.Results The vaccine induced strong cellular and humoral immune response.The titer of antibody in serum was high after inoculation and recognized ROP2 protein antigen expressed in vitro.The lymphocyte phenotype was analyzed.CD4+ T cells proliferated sharply(69.5?3.4)%,and the ratio of CD4+/CD8+ increased considerably by(4.69?1.32)%(P
